This quick guide explains how to safely mix, store, and handle your research peptides.
Before Mixing – Storing Unreconstituted Peptides
| Storage Method | Temperature | Duration | Notes |
|---|---|---|---|
| Freezer | -20°C | Up to 4 years | Best for long-term storage |
| Fridge | 2–8°C | 12–24 months | Ideal for short-to-medium term |
| Room Temp | — | Short term | Stable during shipping and brief exposure. |
Keep sealed: Store vials away from light, moisture, and temperature fluctuations.
Tip: Avoid frequent freezer door openings — temperature swings shorten shelf life.
Reconstitution – Step by Step
1. Warm to Room Temperature
- Remove the peptide and bacteriostatic water from the fridge.
- Let both sit for 15–20 minutes before mixing.
2. Clean the Vial Tops
- Wipe rubber stoppers with an alcohol swab to ensure sterility.
3. Draw Up Bacteriostatic Water
- Use a sterile syringe and needle.
- Add 1–3 mL of bacteriostatic water depending on your desired concentration.
- Use the Bluewell Peptide Calculator for exact dosage calculations.
4. Add Water to the Peptide Vial
- Insert the needle and inject slowly down the side of the vial.
- Avoid squirting directly onto the powder.
5. Dissolve Gently
- Swirl the vial in slow circles — do not shake.
- Wait a few minutes until fully dissolved.
Storage After Reconstitution
| State | Temperature | Shelf Life |
|---|---|---|
| Reconstituted (fridge) | 2–8°C | 3–8 weeks |
| Reconstituted (frozen aliquots) | -20°C | Several months (with cryoprotectant) |
| Unreconstituted (fridge) | 2–8°C | 12–24 months |
| Unreconstituted (freezer) | -20°C | 2–4+ years |
Tip: For long-term use, freeze in small aliquots with cryoprotectant (e.g. glycerol).
Important – Don’t Do This
- Don’t use tap or boiled water.
- Don’t shake vigorously — this damages peptide chains.
- Don’t reuse the same needle between vials.
- Don’t freeze and thaw the same vial multiple times.
Quick Notes
- Some peptides dissolve slower — this is normal.
- Always use bacteriostatic water, not sterile or tap.
- Reconstituted peptides have a short shelf life; dry powder is far more stable.
- When unsure, use the Bluewell Peptide Calculator to confirm your ratios.
